Repeating an induction after the initial induction failed

Previous work had suggested we would get the best results with a re-induction if we held an animal for 3 days after the initial, failed induction. This was difficult because there was only limited space to hold the turtles, especially the SSS which had to be held separately (because of aggression) and required a very large tank.

Once we started using Lutalyse with RES there was only a 6% failure rate with the first induction so turtles to be re-injected were hard to come by. However, the failure rate with Lutalyse alone was 43% in 2016 with RES stressed by malnutrition so we had plenty of turtles to include in a follow-up study that year. When these RES were re-injected with Lutalyse and Sedivet three days after the initial failure we had a success rate of 81% as shown below.

In 2013 we were inducing SSS with a combination of Lutalyse and oxytocin plus Dexdomitor. We re-injected three of these. Two were re-injected after two days and one after three days. They were re-injected with the same doses of Lutalyse, oxytocin, and Dexdomitor as were used for the previous failed induction. All three SSS laid all their eggs with the repeat induction.